Kinetic Studies on the Mechanism and Regulation of Rabbit Liver
نویسنده
چکیده
The interaction of Mg’+, AMP, and fructose 2,6bisphosphate with respect to rabbit liver fructose1,6bisphosphatase was investigated by studying initialrate kinetics of the system at pH 9.5. A rapid-equilibrium Random Bi Bi mechanism is suggested for the rabbit liver enzyme from the kinetic data. Our kinetic findings indicate that Mg’+ and the inhibitor AMP are mutually exclusive in their binding to fructose-1,6bisphosphatase. This probably is the mechanism for AMP regulation of fructose1,6-bisphosphatase and thus, to some extent, gluconeogenesis. A kinetic model for the interaction of these ligands with respect to rabbit liver fructose1,6-bisphosphatase is presented.
منابع مشابه
Kinetic studies on the mechanism and regulation of rabbit liver fructose-1,6-bisphosphatase.
The interaction of Mg2+, AMP, and fructose 2,6-bisphosphate with respect to rabbit liver fructose-1,6-bisphosphatase was investigated by studying initial-rate kinetics of the system at pH 9.5. A rapid-equilibrium Random Bi Bi mechanism is suggested for the rabbit liver enzyme from the kinetic data. Our kinetic findings indicate that Mg2+ and the inhibitor AMP are mutually exclusive in their bin...
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